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Instruments of the Analytical Biopysics Core Facility |
Instruments:
Analytical Ultracentrifuge - Beckman XL-I
The Beckman XL-I is a dual detection analytical ultracentrifuge.
The monochrometer can measure at wavelengths of 190 - 800 nm and scan
with a 0.001 cm resolution.
The interference detection system allows measurement of nonadsorbing species
like typical polymers as well as protein and DNA systems at concentrations
higher than those accessible by off-peak spectroscopy.
As an average of several fringe positions, the interferrometer gives
a strong signal with a very low noise. The camera based detection system
allows for ultrafast scans (< 1sec).
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CD Spectrometer - Olis
The Olis CD spectrometer has all the features of a modern
CD spectrometer. The instrument has computer control for
automated temperature sweeps allowing several melting temperatures
to be determined in one day. The auto-titration attachment
compliments binding as well as chemical denaturation studies.
A standard set of cells (1, 2, 5 & 10 mm path lengths) are available
for general use. Users will be trained in proper care and maintenance
of the instrument and are welcome to borrow the standard cells for
occasional use. Frequent users are strongly encouraged to take charge
of their own cells.
Fluorimeter - Aminco SLM
The Aminco SLM 8000 fluorimeter is a tank of a machine.
T-format detection, Glan-Thompson polarizers, computer controlled
monochrometers, and classic heavy-duty designed optics & mounts
make this instrument robust. The three PMTs with multiple gain electronics
allow for a great deal of sensitivity; easily eclipsing that of
more 'convenient' fluorimeters. It's an older machine and the
computer controller is not sophisticated. However, it can
generate fabulous data. It's currently being retrofitted with a stronger
stirring motor and water-bath for temperature control.
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